Service process
Nanjing Byabscience Biotechnology Co., Ltd. specializes in antibody production and customization services, and has its own rich and unique experience in the development of mouse monoclonal antibodies. In recent years, we have continuously optimized the monoclonal antibody experimental system. From immunization, hybridoma cell fusion to positive hybridoma cell line screening, we have been making in-depth improvements, greatly improving the fusion rate and positive rate. In the process of mouse monoclonal antibody development, whether you provide the antigen or we prepare small molecules, peptides or protein antigens based on the antigen information you provide, we will provide you with the highest quality monoclonal antibodies in the shortest time. For mouse monoclonal antibodies that are difficult to prepare, we have sufficient experience and professional technology to clone mouse antibody genes and prepare genetically engineered antibodies with activity and function using various protein expression platforms. At the same time, in the purification of monoclonal antibodies, we also have professional technology that can purify mouse antibody types including IgG1, IgG2a, IgG2b and IgM.
With our professional and comprehensive R&D team, whether your monoclonal antibody is used for scientific research detection experiments, for the development of diagnostic reagents, or for the preliminary research of antibody drugs, we will combine our many years of R&D experience to maintain good communication with you and our in-depth analysis of the project itself, in order to ensure that the most suitable monoclonal antibody for your experiment is obtained to the greatest extent from the early stage of experimental design.
Final provision: 2-3 hybridoma cell lines and 10ml ascites purified antibodies, 100ul ascites stock solution, 1ml cell supernatant, and give the corresponding identification report (including the following: mouse serum titer, hybridoma construction (fusion rate, positive rate), hybridoma cloning, hybridoma culture supernatant antibody specificity detection, and hybridoma induced ascites ELISA titer detection).
Final guarantee: The monoclonal antibody ascites or hybridoma cell line supernatant provided by the customer has specific binding with the antigen sample provided by the customer through ELISA test; the monoclonal antibody ascites or hybridoma cell line supernatant provided by Western blot test results are positive.
2. Monoclonal antibody customization service types
No. | Name | Service cycle | Price |
BY03A1 | Mouse monoclonal antibody preparation service (customer provides antigen) | 4-6months | Request Quote |
BY03A2 | Mouse monoclonal antibody preparation service (Nanjing Byabscience provides antigens) | 5-7months | Request Quote |
BY03A3 | Rat monoclonal antibody preparation service (customer provides antigen) | 4-6months | Request Quote |
BY03A4 | Rat monoclonal antibody preparation service (Nanjing Byabscience provides antigen) | 5-7months | Request Quote |
BY03B1 | Mouse rapid monoclonal antibody preparation service (customer provides antigen) | 8-10weeks | Request Quote |
BY03B2 | Mouse rapid monoclonal antibody preparation service (Nanjing Byabscience provides antigens) | 9-14weeks | Request Quote |
BY03B3 | Rat rapid monoclonal antibody preparation service (customer provides antigen) | 12-14weeks | Request Quote |
BY03B4 | Rat rapid monoclonal antibody preparation service (Nanjing Byabscience provides antigens) | 14-18weeks | Request Quote |
BY03C | Anti-idiotypic monoclonal antibody preparation service (mouse) | consult | Request Quote |
BY03D | Modified monoclonal antibody preparation service (Nanjing Byabscience provides antigens) | consult | Request Quote |
BY03E | Preparation of monoclonal antibodies in serum-free medium | 4-6months | Request Quote |
3. Technical advantages
① The latest service package: integrated services of antibody preparation, antibody purification, and antibody screening; we can provide a variety of purification methods, such as ammonium sulfate octanoate, Protein A/G, antigen affinity chromatography, etc.; antibody screening saves you time, and we can screen antibody pairs and verify the recognition of natural samples according to customer needs, so that customers have no worries.
② Diversified customized services: we can provide mouse and rat monoclonal antibody service platforms, semi-solid mode rapid preparation of monoclonal antibody service platforms, and serum-free medium monoclonal antibody preparation service platforms. Customers can flexibly choose according to their needs.
③ Diversity of antigens and immune types: Boyan has a powerful protein service platform. Customers can choose Boyan to provide antigens. There are a variety of expression systems for you to choose from, such as mammalian proteins that are closer to the natural conformation, which can make your antibody quality higher; in addition, we immunize at least 5 mice/rats for each project, using a variety of immune pathways, and a multi-pronged approach to ensure the acquisition of higher-quality monoclonal antibodies.
④ Large-scale production services: we can provide the most professional large-scale production services for monoclonal antibodies to meet the needs of diagnostic-level mass production.
⑤ High quality: We are specialized in antibody production and customization services, and have established a series of related technical platforms and animal breeding bases with the antibody platform as the core. The monoclonal antibody products we provide are completed by professionals who have been engaged in antibody production for many years. The technical route is mature, the product quality is stable and reliable, and the sensitivity is extremely high. So far, this platform has successfully prepared a variety of diagnostic reagent-level monoclonal antibody raw materials, which are well received by downstream customers.
Affordable price: Monoclonal antibodies have high specificity and good sensitivity. Our service price is preferential, which can provide you with high-quality products while saving costs.
3. Service Examples
We work closely with many well-known scientific research reagent companies, diagnostic reagent companies, and biopharmaceutical companies around the world. We continue to develop monoclonal antibodies that can meet the specific application requirements of customers, such as scientific research antibodies used in ELISA, IHC, IP, western blot and other technical platforms; we can develop monoclonal antibody pairs suitable for customers' specific application requirements, so that they can be used in diagnostic antibodies for platforms such as ELISA, colloidal gold test strips, chemiluminescent reagents or immunomagnetic beads; we can also develop highly sensitive small molecule and peptide monoclonal antibodies for customers; professional, efficient and economical are our solemn promises to customers.
1. Customization of diagnostic-grade monoclonal antibodies
① A monoclonal antibody developed by our company for a customer screened 4 different epitopes, all of which can be paired; and was successfully applied to the development of ELISA, colloidal gold, and latex immunoenhanced turbidimetry kits .
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